PPP2R4 Antibody PA5-19136 from Thermo Fisher Scientific

Supplier Images

WB

Western blot was performed using Anti-PPP2R4 Polyclonal Antibody (Product # PA5-19136) and a 38 kDa band corresponding to PPP2R4 was observed across all the tissues and cell line tested. The expression of PPP2R4 is reported to be low in Skeletal Muscle. Whole cell extracts (30 µg) of HeLa (Lane 1), tissue extracts (30 µg lysate) of Mouse Brain (Lane 2), Mouse Liver (Lane 3), Mouse Lung (Lane 4) and Mouse Skeletal Muscle (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 µg/mL) and detected by chemiluminescence with Rabbit anti-Goat IgG Heavy Chain, Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
ICC/IF

Immunofluorescence analysis of PPP2R4 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PPP2R4 Goat Polyclonal Antibody (Product # PA5-19136) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27012) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing cytoplasmic and nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
PPP2R4 Antibody

Supplier Page from
Thermo Fisher Scientific for
PPP2R4 Antibody

The PPP2R4 Antibody from Thermo Fisher Scientific is a Goat Polyclonal antibody to protein phosphatase 2 protein activator, and PTPA. This antibody recognizes Homo Sapiens, Human, Mouse, and Rat antigen. The PPP2R4 Antibody has been validated for the following applications: Immunohistochemistry, and Western Blot.

WB

ICC/IF

PPP2R4 Antibody

PPP2R4 Antibody from Thermo Fisher Scientific

Thermo Fisher Scientific

Reviews

PPP2R4 Antibody from Thermo Fisher Scientific

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map